Hoechst 33258 [23491-44-3]

Biological Activity

Hoechst 33258 (dibiphenimide) can bind dsDNA, with a maximum excitation/emission wavelength of 352 nm/461 nm (after binding with DNA), giving blue fluorescence, which is often used to observe the nucleus and nucleic acid. Store at room temperature away from light.

Chemical Information

Molecular Weight	424.50
Formula	C25H24N6O
CAS Number	23491-44-3
Solubility (25°C)	DMSO ≥ 20 mg/mL
Storage	-20°C, protect from light, dry, sealed

References

[1] Jos M R Flauzino, et al. Bioelectrochemistry. DNA electrochemical biosensor for detection of Alicyclobacillus acidoterrestris utilizing Hoechst 33258 as indicator

[2] Xin-Xing Zhang, et al. Commun Biol. DNA minor-groove binder Hoechst 33258 destabilizes base-pairing adjacent to its binding site

[3] Karen Yu Amirbekyan, et al. J Biomol Struct Dyn. Fluorescence anisotropy studies on the Hoechst 33258-DNA interaction: the solvent effect

[4] Poghos O Vardevanyan, et al. J Biomol Struct Dyn. Spectroscopic study of interaction of various GC-content DNA with Hoechst 33258 depending on Na + concentration

[5] N P Bazhulina, et al. J Biomol Struct Dyn. Binding of Hoechst 33258 and its derivatives to DNA