Goat Anti-Smooth muscle alpha-actin Antibody
Referência AF2010a
Tamanho : 100ug
Marca : Abcepta
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Goat Anti-Smooth muscle alpha-actin Antibody
Peptide-affinity purified goat antibody
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AF2010a (2.5 µg/ml) staining of paraffin embedded Human Lung (top) and Mouse Lung (bottom). Trypsin-mediated antigen retrieval, HRP-staining. Bar in right lower corner represents 50um. Data provided by Dr L. Marsh, Ludwig Boltzmann Institute for Lung Vascular Research, Graz, Austria
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AF2010a staining (0.5 µg/ml) of Human Duodenum lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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EB06450 (1µg/ml) staining of HeLa (A) and (2ug/ml) NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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EB06450 (0.3µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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EB06450 (1µg/ml) staining of HeLa (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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EB06450 (1µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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EB06450 (1µg/ml) staining of HeLa (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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EB06450 (0.3µg/ml) staining of Human Heart (A) and (2µg/ml) Mouse Duodenum (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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EB06450 (1µg/ml) staining of HeLa (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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EB06450 (1µg/ml) staining of Human Heart (A), (0.1ug/ml) Mouse Duodenum (B) and (0.03ug/ml) Rat Heart (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
AF2010a (2.5 µg/ml) staining of paraffin embedded Human Lung (top) and Mouse Lung (bottom). Trypsin-mediated antigen retrieval, HRP-staining. Bar in right lower corner represents 50um. Data provided by Dr L. Marsh, Ludwig Boltzmann Institute for Lung Vascular Research, Graz, Austria
Application 
| WB, IHC-P, Pep-ELISA |
|---|---|
| Primary Accession | P62736 |
| Other Accession | NP_001135417, 59 |
| Reactivity | Human, Mouse, Rat, Pig |
| Predicted | Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 42009 Da |
| Gene ID | 59 |
|---|---|
| Other Names | Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS |
| Dilution | WB~~1:1000 IHC-P~~N/A Pep-ELISA~~N/A |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-Smooth muscle alpha-actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ACTA2 |
|---|---|
| Synonyms | ACTSA, ACTVS |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Cellular Location | Cytoplasm, cytoskeleton. |
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