GBRR1 Human Recombinant Protein, Synthetic Nanodisc
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SKU
TP728593M
Recombinant Human GBRR1 full length protein-Synthetic Nanodisc, 100 ug
Specifications
Product Data
Species
Human
Expression Host
HEK293
Tag
C-Flag & Strep Tag
Predicted MW
55.9 kDa
Concentration
Please refer to the Certificate of Analysis (COA) for the lot-specific concentration before lyophilization.
Buffer
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Do not use solvents with pH lower than 6.5 in subsequent experiments.
Reconstitution Method
1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube. 2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000 3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL) 4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample.
Storage
Store at -20°C to -80°C for 12 months in lyophilized form.
Stability
After reconstitution, if not intended for use within a month, aliquot and store at -80°C . Avoid repeated freezing and thawing.
The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. The subunit encoded by this gene is expressed in several non-neuronal tissues including the uterus and ovaries. This subunit can assemble with known GABA A receptor subunits, and the presence of this subunit alters the sensitivity of recombinant receptors to modulatory agents such as pregnanolone. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, May 2014
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