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- Product Name
- FineTest®488 Anti-Human CD127/IL-7RA Antibody(FN195D)
- Catalogue No.
- F488-30033
- Form
- liquid
- Conjugation
- FineTest®488
- Conjugation Information
- FineTest® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).
- Clonality
- Monoclonal
- Isotype
- IgG1, κ
- Clone ID
- FN195D
- Storage
- PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Alternative Names
- CD127|CDw127|IL-7RA|IL-7Rα|Interleukin-7 receptor subunit alpha antibody
- UniProt ID
- P16871
Application
- Tested Applications
- FC
- Recommended dilution
- Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Validated Images
Human peripheral blood lymphocytes are stained with APC Anti-Human CD3 Antibody and FineTest® 488 Anti-Human CD127/IL-7RA Antibody(A019D5) (Left). Lymphocytes are stained with APC Anti-Human CD3 Antibody and FineTest® 488 Mouse IgG1, κ Isotype Control (Right). - Background
- CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.