EDN3 ELISA Kit (Mouse)
Referência OKEH01505
Tamanho : 96Wells
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
EDN3 ELISA Kit (Mouse) (OKEH01505)
| Datasheets/Manuals | Printable datasheet for EDN3 ELISA Kit (Mouse) (OKEH01505) |
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| Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology EDN3 ELISA Kit (Mouse) (OKEH01505) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EDN3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EDN3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EDN3 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 31.2-2000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.9% (n = 20) Mean Inter-assay CV%: < 11.8% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 15.72 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Mouse Endothelin-3 | ||||||||||||||||||||||
| Publications | Endothelin 3/EDNRB signaling induces thermogenic differentiation of white adipose tissue | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Edn3 |
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| Gene Full Name | endothelin 3 |
| Alias Symbols | endothelin-3, ET-3, ls, PPET3, preproendothelin-3, tmgc48. |
| NCBI Gene Id | 13616 |
| Protein Name | Endothelin-3 |
| Description of Target | Endothelins are endothelium-derived vasoconstrictor peptides. |
| Uniprot ID | P48299 |
| Protein Accession # | NP_031929.1 |
| Nucleotide Accession # | NM_007903.5 |
| Protein Size (# AA) | 214 |
| Molecular Weight | 23 kDa |
