Donkey anti-Rat IgG (H&L), HRP conjugated
Referência AS10947
Tamanho : 1mg
Marca : Agrisera
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Donkey anti-Rat IgG (H&L), HRP conjugated
Product no: AS10 947
AS10 947 |Clonality: Polyclonal | Host: Donkey | Reactivity: Rat IgG (H&L)
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| Host: | Donkey |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified donkey IgG. |
| Format: | Lyophilized |
| Quantity: | 1 mg |
| Reconstitution: | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily |
| Storage: | Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
| Recommended dilution: | The optimal working dilution should be determined by the investigator |
| Additional information: | This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins by immunoelectrophoresis. Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase. |
| Background: | Donkey anti-rat IgG (H&L), is a secondary HRP (horse radish peroxidase) conjugated antibody which binds to rat IgGs in immunological assays. |
| Selected references: | Bui et al. (2020). Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor. doi.org/10.1101/2020.02.12.945923 BioRxiv. |