DNA and RNA Lysis and Binding Buffer
Catalog Number: B2019321 (50 mL)
DNA and RNA lysis and binding buffers are used in molecular biology to extract and purify nucleic acids from cells. The lysis buffer breaks open cells to release nucleic acids, while the binding buffer facilitates their binding to a solid phase for purification. These buffers are crucial for successful nucleic acid extraction. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.
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Product Description
DNA and RNA Lysis and Binding Buffer
Catalog number: B2019321
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 mL
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: RT
Keywords: DNA and RNA Lysis and Binding Buffer
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.
References:
1: Tsuji Y. Optimization of Biotinylated RNA or DNA Pull-Down Assays for Detection of Binding Proteins: Examples of IRP1, IRP2, HuR, AUF1, and Nrf2 Int J Mol Sci. 2023 Feb 10;24(4):3604.
2: Holmberg RC, Gindlesperger A, Stokes T, Brady D, Thakore N, Belgrader P, Cooney CG, Chandler DP. High-throughput, automated extraction of DNA and RNA from clinical samples using TruTip technology on common liquid handling robots J Vis Exp. 2013 Jun 11;(76):e50356.
3: Kreuter J, Bica-Schröder K, Pálvölgyi ÁM, Krska R, Sommer R, Farnleitner AH, Kolm C, Reischer GH. A novel ionic liquid-based approach for DNA and RNA extraction simplifies sample preparation for bacterial diagnostics Anal Bioanal Chem. 2024 Dec;416(29):7109-7120.
4: Liu M, Du M, Yu J, Qian Z, Gao Y, Pan W, Zhao X, Wang M, Li H, Zheng J, Huang Q, Wang LM, Xiao H. CEBPA mutants down-regulate AML cell susceptibility to NK-mediated lysis by disruption of the expression of NKG2D ligands, which can be restored by LSD1 inhibition Oncoimmunology. 2022 Jan 5;11(1):2016158.
5: Ståhlberg A, Thomsen C, Ruff D, Åman P. Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell Clin Chem. 2012 Dec;58(12):1682-91.
6: Gomes AG, Azevedo AM, Aires-Barros MR, Prazeres DM. Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads J Chromatogr A. 2011 Dec 2;1218(48):8629-37.
7: Wielinga PR, de Heer L, de Groot A, Hamidjaja RA, Bruggeman G, Jordan K, van Rotterdam BJ. Evaluation of DNA extraction methods for Bacillus anthracis spores spiked to food and feed matrices at biosafety level 3 conditions Int J Food Microbiol. 2011 Nov 1;150(2-3):122-7.
8: Michel AD, Chessell IP, Hibell AD, Simon J, Humphrey PP. Identification and characterization of an endogenous P2X7 (P2Z) receptor in CHO-K1 cells Br J Pharmacol. 1998 Nov;125(6):1194-201.
9: Nishizawa Y, Komori N, Usukura J, Jackson KW, Tobin SL, Matsumoto H. Initiating ocular proteomics for cataloging bovine retinal proteins: microanalytical techniques permit the identification of proteins derived from a novel photoreceptor preparation Exp Eye Res. 1999 Aug;69(2):195-212.
10: Li H, Dunbar JC, Dhabuwala CB. Expression of cAMP-responsive element modulator (CREM) in rat testes following chronic cocaine administration J Environ Pathol Toxicol Oncol. 2003;22(2):111-6.
Products Related to DNA and RNA Lysis and Binding Buffer can be found at Chemicals
Additional Information
| Weight | 2.6 oz |
|---|---|
| Dimensions | 3.3 × 1.2 × 1.2 in |