COL3A1 ELISA Kit (Human)
Referência OKEH00269
Tamanho : 96Wells
Marca : Aviva Systems Biology
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COL3A1 ELISA Kit (Human) : 96 Wells (OKEH00269)
| Datasheets/Manuals | Printable datasheet for COL3A1 ELISA Kit (Human) : 96 Wells (OKEH00269) |
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| COA Datasheet | Printable COA datasheet for OKEH00269 |
| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology COL3A1 ELISA Kit (Human) (OKEH00269) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COL3A1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for COL3A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample COL3A1 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 3.12-200ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 3.9% (n = 20) Mean Inter-assay CV%: < 7.1% (n = 20) | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 1.2 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Collagen alpha-1(III) chain | ||||||||||||||||||||||
| Publications | Dysregulation of extracellular matrix and Lysyl Oxidase in Ehlers-Danlos syndrome type IV skin fibroblasts Dysregulation of extracellular matrix and Lysyl Oxidase in Ehlers-Danlos Syndrome Type IV skin fibroblasts Preprint — no PubMed record available | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | COL3A1 |
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| Gene Full Name | collagen type III alpha 1 chain |
| Alias Symbols | alpha-1 type III collagen, alpha1 (III) collagen, collagen alpha-1(III) chain, collagen, fetal, collagen, type III, alpha 1, EDS4A, EDSVASC, Ehlers-Danlos syndrome type IV, autosomal dominant, PMGEDSV. |
| NCBI Gene Id | 1281 |
| Protein Name | Collagen alpha-1(III) chain |
| Description of Target | Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12. |
| Uniprot ID | P02461 |
| Protein Accession # | NP_000081.1 |
| Nucleotide Accession # | NM_000090.3 |
| Protein Size (# AA) | 1466 |
| Molecular Weight | 139 kDa |
