Cholesterol oxidase [9028-76-6]

Cholesterol oxidase is a flavoenzyme that catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one. ChOx, an important virulence factor for Mycobacterium tuberculosis (Mtb), competitively inhibits TLR2 thereby inducing enhanced reactive oxygen species production by macrophages.

HCT116 cells:64.6%(cell viability inhibition at 23 UmL−1), HeLa cells:62.9%(cell viability inhibition at 23 UmL−1), MCF-7 cells:75.3%(cell viability inhibition at 23 UmL−1), HepG2 cells:69.4%(cell viability inhibition at 23 UmL−1)

Using the MTT assay, Cholesterol oxidase (23 U/mL) showed an inhibition rate of 62.9%-78.7% on cancer cells such as HepG-2 and HCT-116, and an inhibition rate of 57.2% on normal WI-38 cells. Additionally, Cholesterol oxidase can be used to detect cholesterol in serum and food samples (incubated at 37°C for 10 minutes) as well as cholesterol in cells/tissues (using the AmplexRed fluorescence method) [1,2].

H2O: 20 mg/mL, Sonication is recommended.