Canine kidney injury molecule 1(Kim-1) ELISA Kit
Referência CSB-E13035c-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Intra-assay Precision (Precision within an assay): CV%<8% | |||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||
Three samples of known concentration were tested in twenty assays to assess. | |||||
To assess the linearity of the assay, samples were spiked with high concentrations of canine Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 91 | ||||
Range % | 86-95 | |||||
1:2 | Average % | 102 | ||||
Range % | 97-107 | |||||
1:4 | Average % | 91 | ||||
Range % | 85-97 | |||||
1:8 | Average % | 97 | ||||
Range % | 91-103 | |||||
The recovery of canine Kim-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 87 | 80-93 | ||||
EDTA plasma (n=4) | 90 | 83-96 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ||||||
ng/ml | OD1 | OD2 | Average | Corrected | ||
20 | 2.449 | 2.563 | 2.506 | 2.348 | ||
10 | 1.595 | 1.538 | 1.567 | 1.409 | ||
5 | 1.001 | 1.052 | 1.027 | 0.869 | ||
2.5 | 0.687 | 0.648 | 0.668 | 0.510 | ||
1.25 | 0.463 | 0.473 | 0.468 | 0.310 | ||
0.625 | 0.306 | 0.314 | 0.310 | 0.152 | ||
0.312 | 0.221 | 0.232 | 0.227 | 0.069 | ||
0 | 0.154 | 0.161 | 0.158 |
| ||
and FAQs
This Dog Kim-1 ELISA Kit was designed for the quantitative measurement of Dog Kim-1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.78 ng/mL.
Citations
- RE Akhigbe,Life Sciences,2020
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