BMP2 ELISA Kit (Chicken)
Referência OKEH07223
Tamanho : 96T
Marca : Aviva Systems Biology
Contactar o distribuidor local :
Telefone : +1 850 650 7790
BMP2 ELISA Kit (Chicken) (OKEH07223)
| Datasheets/Manuals | Printable datasheet for BMP2 ELISA Kit (Chicken) (OKEH07223) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology BMP2 ELISA Kit (Chicken) (OKEH07223) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMP2 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 115% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: <=6.2% (n = 20) Mean Inter-assay CV%: <=8.8% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Function: Negatively regulates the structure and function of the limb apical ectodermal ridge. | ||||||||||||||||||||||
| :: | Subcellular Location: Secreted | ||||||||||||||||||||||
| :: | Unigene: Gga.3950 String: 9031.ENSGALP00000014334 | ||||||||||||||||||||||
| :: | Pfam: PF00019 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sensitivity | 0.078ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Chicken Bone morphogenetic protein 2 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | BMP2 |
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| Alias Symbols | BMP-2, BMP-2 |
| Protein Name | Bone morphogenetic protein 2 |
| Description of Target | Negatively regulates the structure and function of the limb apical ectodermal ridge. |
| Uniprot ID | Q90751 |