BCIP/NBT Alkaline Phosphatase Color Development Kit

Alkaline phosphatase (AP/ALP/AKP/ALKP/ALPase/Alk Phos) is a group of isoenzymes that can generate phosphate ions and free hydroxyl groups by hydrolyzing phosphate monoesters to remove phosphate groups on substrate molecules, and its dephosphorylation substrates include nucleotides, proteins and alkaloids, and are most effective under alkaline conditions. Alkaline phosphates have hydrolase activities, such as the common calf intestinal alkaline phosphatase (CIAP/CIP), which is widely used in the detection of labeled proteins and nucleic acids such as secondary antibodies, as well as for dephosphorylation (dephosphorylation) of DNA or RNA 5' and 3' ends, especially the 5' end of plasmids to avoid plasmid self-linking.

Nitroblue (5-Bromo-4-chloro-3-indolyl phosphate, BCIP) is one of the best substrate combinations for alkaline phosphatase, and Nitroblue tetrazolium chloride (NBT) is a dark blue amorphous microsoluble substance. Catalyzed by alkaline phosphatase, BCIP is hydrolyzed to produce a strongly reactive product that reacts with NBT to form the insoluble dark blue to blue-violet compound NBT-formazan. Strong colors can be observed visually, are very stable, and do not fade when exposed to light.

This kit can be used for immunohistochemical chromography, Western and other membrane chromogenic and identification of induced pluripotent stem cell iPS.