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> Azithromycin [83905-01-5]

Azithromycin [83905-01-5]

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Referência HY-17506-200mg

Tamanho : 200mg

Marca : MedChemExpress

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Azithromycin is a macrolide antibiotic useful for the treatment of a number of bacterial infections.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

Azithromycin Chemical Structure

Azithromycin Chemical Structure

CAS No. : 83905-01-5

Size Prix Stock Quantité
Échantillon gratuit (0.1 - 0.5 mg)   Appliquer maintenant  
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 73 En stock
Solution
10 mM * 1 mL in DMSO USD 73 En stock
Solid
50 mg USD 66 En stock
100 mg USD 99 En stock
200 mg USD 154 En stock
500 mg USD 264 En stock
1 g   Obtenir un devis  
5 g   Obtenir un devis  

* Veuillez sélectionner la quantité avant d'ajouter des articles.

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Based on 21 publication(s) in Google Scholar

Other Forms of Azithromycin:

Azithromycin Related Antibodies

Top Publications Citing Use of Products

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Voir tous les produits spécifiques à Isoform Antibiotic:

Voir toutes les isoformes

Voir tous les produits spécifiques à Isoform Parasite:

Voir toutes les isoformes

  • Activité biologique

  • Protocole

  • Pureté et documentation

  • Références

  • Avis client

Description

Azithromycin is a macrolide antibiotic useful for the treatment of a number of bacterial infections.

IC50 & Target

Macrolide

 

In Vitro

Azithromycin (2 μM) augments rhinovirus-induced IFNβ expression in primary bronchial epithelial cells from asthmatics, which is associated with over-expression of RIG-I like receptors and repression of viral replication. Knockdown of MDA5, but not knockdown of RIG-I, diminishes azithromycin (2 μM)-enhanced viral-induced IFNβ expression in asthmatic primary bronchial epithelial cells[1]. Azithromycin specifically reduces MMP-9 mRNA and protein levels without affecting NF-κB in endotoxin-challenged monocytic THP-1 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Azithromycin Related Antibodies
In Vivo

Azithromycin (50 mg/kg) has no effect on bronchoalveolar lavage inflammatory parameters and LDH levels in a mouse model of asthma exacerbation. Azithromycin induces neither general inflammatory parameters nor LDH release in a mouse model of asthma exacerbation, and augments expression of interferon-stimulated genes and the pattern recognition receptor MDA5 but not RIG-I in exacerbating mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Essai clinique
Masse moléculaire

748.98

Formule

C38H72N2O12
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@H]([C@@H]([C@@](C(O[C@@H]([C@@](C)(O)[C@@H]1O)CC)=O)(00)C)O[C@@](O[C@@H](C)[C@@H]2O)(00)C[C@@]2(C)OC)[C@H]([C@](O)(C[C@H](CN([C@@H]1C)C)C)C)O[C@@](O[C@H](C)C[C@@H]3N(C)C)(00)[C@@H]3O
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvant et solubilité
In Vitro: 

DMSO : ≥ 100 mg/mL (133.51 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3351 mL 6.6757 mL 13.3515 mL
5 mM 0.2670 mL 1.3351 mL 2.6703 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.34 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (3.34 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation

Purity: ≥98.0%

COA (257 KB) HNMR (300 KB)

Instruction de manipulation (2659 KB)
Références
Test cellulaire
[2]

THP-1 cells (106 cells in 1 mL RPMI medium, without antibiotics, growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next, 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO2) the supernatants and cell pellets are collected (1200 rpm, 5 min). THP-1 cell viability is tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm.

MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
Références

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3351 mL 6.6757 mL 13.3515 mL 33.3787 mL
5 mM 0.2670 mL 1.3351 mL 2.6703 mL 6.6757 mL
10 mM 0.1335 mL 0.6676 mL 1.3351 mL 3.3379 mL
15 mM 0.0890 mL 0.4450 mL 0.8901 mL 2.2252 mL
20 mM 0.0668 mL 0.3338 mL 0.6676 mL 1.6689 mL
25 mM 0.0534 mL 0.2670 mL 0.5341 mL 1.3351 mL
30 mM 0.0445 mL 0.2225 mL 0.4450 mL 1.1126 mL
40 mM 0.0334 mL 0.1669 mL 0.3338 mL 0.8345 mL
50 mM 0.0267 mL 0.1335 mL 0.2670 mL 0.6676 mL
60 mM 0.0223 mL 0.1113 mL 0.2225 mL 0.5563 mL
80 mM 0.0167 mL 0.0834 mL 0.1669 mL 0.4172 mL
100 mM 0.0134 mL 0.0668 mL 0.1335 mL 0.3338 mL
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Azithromycin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Azithromycin83905-01-5CP 62993CP62993CP-62993BacterialAutophagyAntibioticParasiteInhibitorinhibitorinhibit

Bulk Inquiry

Inquiry Information

Nom du produit:
Azithromycin
Cat. No.:
HY-17506
Quantité:
MCE Japan Authorized Agent:

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