Anti-Neurofilament M (NEFM)
Referência OAPC00028
Tamanho : 100ul
Marca : Aviva Systems Biology
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Anti-Neurofilament M (NEFM) (OAPC00028)
| Datasheets/Manuals | Printable datasheet for OAPC00028 |
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| Predicted Species Reactivity | Human, Mouse, Rat |
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| Product Format | Liquid containing 10 mM sodium azide. |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Host | Mouse |
| Application | WB, IF |
| Reconstitution and Storage | Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles. |
| Immunogen | Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli. |
| Purification | Unpurified, concentrated culture supernatant. |
| Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
| Specificity | Specific for the ~145k neurofilament M protein. |
| Application Info | The antibody has been directly tested for reactivity in chicken, human, mouse and rat tissues. Suggested antibody dilutions: WB ~ 1:1000 IF ~ 1:100 |
| Reference | 1.Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62. 2.Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60. |
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| Gene Symbol | NEFM |
| Alias Symbols | Nfm, Nef3 |
| NCBI Gene Id | 24588 |
| Protein Name | Neurofilament medium polypeptide |
| Description of Target | Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2). |
| Uniprot ID | P12839 |
| Protein Accession # | NP_058725.1 |
| Molecular Weight | ~145kDa |
