Anti-Mouse I-A/I-E (MHC Class II) [Clone M5/114.15.2] - PE
Referência H274-100
Tamanho : 100ug
Marca : Leinco Technologies
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AntiMouse IA/IE (MHC Class II) [Clone M5/114.15.2] — PE
AntiMouse IA/IE (MHC Class II) [Clone M5/114.15.2] — PE
Product No.: H274
Clone M5/114.15.2 Target IA/IE MHC class II Formats AvailableView All Product Type Monoclonal Antibody Isotype IgG2b Applications FC |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen C57BL/6 Spleen Cells Product Concentration 0.2 mg/ml Formulation This Rphycoerythrin (RPE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This Rphycoerythrin (RPE) conjugate is stable when stored at 28°C. Do not freeze. Country of Origin USA Shipping Next Day 28°C Excitation Laser Blue Laser (488 nm) and/or Green Laser (532 nm)/YellowGreen Laser (561 nm) RRIDAB_2892898 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this Clone M5/114.15.2 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone M5/114.15.2 recognizes an epitope on the mouse MHC class II molecules IAb, IAd, IAq, IEd, and IEk. Clone M5/114.15.2 does not react with IAf, IAk, or IAs. Background MHC class II antibody, clone M5/114.15.2, recognizes the major histocompatibility complex (MHC) class II molecules IAb, IAd, IAq, IEd, and IEk. MHC class II is constitutively expressed on professional antigenpresenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation1. MHC class II consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain2. The Nterminal α1 and β1 domains form the antigenbinding groove, which binds 1325 aa peptides derived from exogenous antigens3. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptideMHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling4. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation5 and can transduce signals into T cells, enhancing T cell proliferation and activity6. Antigen Distribution IAb/d/q and IEd/k are expressed on antigenpresenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells from mice of the IAb/d/q and IEd/k haplotypes. Ligand/Receptor CD3/TCR, CD4 PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity References & Citations1. Holling TM, et al. (2004) Hum Immunol. 65(4):28290 2. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):1061825 3. Wieczorek M, et al. (2017) Front Immunol. 8:292 4. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):1691616921 5. Barnaba V, et al. (1994) Eur J Immunol. 24(1):715 6. Di Rosa F, et al. (1993) Hum Immunol. 38(4):25160 |