| Short Description : | Rabbit polyclonal antibody anti-HRAS (100-150) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Applications: | ELISA/IP/WB |
| Host: | Rabbit |
| Reactivity: | Chicken/Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Biotin |
| Concentration: | 1.00-1.25 µg/µl |
| Purification: | Affinity Purified |
| Dilution Range: | WB: 1:500ELISA: 1:10, 000IP: 1:150 |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
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| Gene ID: | |
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| Immunogen: | Synthetic peptide taken within amino acid region 100-150 on human GTPase HRAS protein. |
| Immunogen Region: | 100-150 |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. S-nitrosylated.critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). Fatty-acylated at Lys-170. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL. Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to inhibit Ras signaling. |
| Function | Involved in the activation of Ras protein signal transduction. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. |
| Protein Name | Gtpase HrasH-Ras-1Ha-RasTransforming Protein P21C-H-RasP21ras Cleaved Into - Gtpase Hras - N-Terminally Processed |
| Database Links | |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideGolgi ApparatusGolgi Apparatus MembraneThe Active Gtp-Bound Form Is Localized Most Strongly To Membranes Than The Inactive Gdp-Bound FormShuttles Between The Plasma Membrane And The Golgi ApparatusIsoform 2: NucleusCytoplasmPerinuclear RegionColocalizes With Rack1 To The Perinuclear Region |
| Alternative Antibody Names | Anti-Gtpase Hras antibodyAnti-H-Ras-1 antibodyAnti-Ha-Ras antibodyAnti-Transforming Protein P21 antibodyAnti-C-H-Ras antibodyAnti-P21ras Cleaved Into - Gtpase Hras - N-Terminally Processed antibodyAnti-HRAS antibodyAnti-HRAS1 antibody |
Information sourced from Uniprot.org