Anti-H3T6pK9me2 | Histone H3 (dimethylated Lys9, p Thr6)
Referência AS163175
Tamanho : 50ug
Marca : Agrisera
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Anti-H3T6pK9me2 | Histone H3 (dimethylated Lys9, p Thr6)
Product no: AS16 3175
AS16 3175 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
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| Immunogen: | KLH-conjugated synthetic peptide |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified serum. |
| Format: | Liquid |
| Quantity: | 50 µg |
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) |
| Recommended dilution: | 2-5 µg/million cells (ChIP), 1 : 100 (IF), 1 : 200 (IHC), 1 : 500 (WB) |
| Expected | apparent MW: | 15 kDa |
| Confirmed reactivity: | Human |
| Predicted reactivity: | Caenorhabditis elegans, Chicken, Drosophila melanogaster, Human, Mouse, Plant, Rat, Xenopus sp. |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
application example Chromatin Immunoprecipitation using anti-H3T6pK9me2 antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T6pK9me2 antibody to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qPCR and normalized to total input. |
| Additional information: | This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol |
| Background: | H3K9me2 is a histone post-translational modification that is enriched in transcriptionally inactive regions. It is also important in cellular differentiation and maturation. Phosphorylation of histone H3 is also associated with mitosis and meiosis, and seems to be related to developmental changes. |