Anti-CD40 Antibody-APC labled

Description：Armenian Hamster monoclonal antibody APC labled to CD40Immunogen：(BALB/c x NZB)F1 mouse-derived lymphoma WEHI 231Purification：The antibody was purified by affinity chromatography.Specificity：Recognizes mouse CD40Clonality：Monoclonal (clone: 1C10)Conjugation：APCForm：Rat IgG2a kappa. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.Dilution：10 μl / assayGene Symbol：CD40Alternative Names：TNFRSF5; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD40 Entrez Gene (Mouse)： 21939; SwissProt (Mouse)： P27512; Directions for Use：1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Storage/Stability：Shipped and store at 4°C for one year. Do not freeze.