Anti-CD11b Antibody-PerCP/Cy5.5 labled

Description：Rat monoclonal antibody PerCP-Cyanine5.5 labled to CD11bImmunogen：C57BL/10 splenocytesPurification：The antibody was purified by affinity chromatography.Specificity：Recognizes mouse CD11bClonality：Monoclonal (clone: M1/70)Conjugation：PerCP-Cyanine5.5Form：Rat IgG2b kappa. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.Dilution：10 μl / assayGene Symbol：ITGAMAlternative Names：CD11B; CR3A; Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD11b Entrez Gene (Human)： 3684; Entrez Gene (Mouse)： 16409; SwissProt (Human)： P11215; SwissProt (Mouse)： P05555; Directions for Use：1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Platform：Ex/Em = 481/690 nm Storage/Stability：Shipped and store at 4°C for one year. Do not freeze.