Anti-CD102 Antibody-FITC labled

Referência CFE8841-100

Tamanho : 100Assays

Marca : Cohesion Biosciences

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Product Name:Anti-CD102 Antibody-FITC labled
Cat No:CFE8841
Source:Rat
Reactivity:M
Applications:IF, FC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description:Rat monoclonal antibody FITC labled to CD102
Immunogen:COS cells transfected with mouse ICAM-2 cDNA
Purification:The antibody was purified by affinity chromatography.
Specificity:Recognizes mouse CD102
Clonality:Monoclonal (clone: 3C4)
Conjugation:FITC
Form:Rat IgG2a kappa. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
Dilution:10 μl / assay
Gene Symbol:ICAM2
Alternative Names:Intercellular adhesion molecule 2; ICAM-2; CD102
Entrez Gene (Mouse): 15896;
SwissProt (Mouse): P35330;
Directions for Use:1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Storage/Stability:Shipped and store at 4°C for one year. Do not freeze.