Anti-53BP1 Antibody

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Tamanho : 30ul

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Western blot analysis of 53BP1 expression in HeLa (A), COS7 (B), CT26 (C), PC12 (D) whole cell lysates. (Predicted band size: 213; 214 kD; Observed band size: 255 kD)
Immunohistochemical analysis of 53BP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of 53BP1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Name:	Anti-53BP1 Antibody
Cat No:	CPA3348
Source:	Rabbit
Reactivity:	H, M, R
Applications:	WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to 53BP1

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human 53BP1. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Specificity：Recognizes endogenous levels of 53BP1 protein.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene Symbol：TP53BP1

Alternative Names：Tumor suppressor p53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1

Entrez Gene (Human)： 7158;

SwissProt (Human)： Q12888;

SwissProt (Mouse)： P70399;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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