ANGPT2 ELISA Kit (Bovine)
Referência OKEH06577
Tamanho : 96Wells
Marca : Aviva Systems Biology
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ANGPT2 ELISA Kit (Bovine) (OKEH06577)
| Datasheets/Manuals | Printable datasheet for ANGPT2 ELISA Kit (Bovine) (OKEH06577) |
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| Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Angpt2 ELISA Kit (Bovine) (OKEH06577) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Angpt2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Angpt2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Angpt2 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 78-5000pg/ml | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12% | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
| Sensitivity | 38 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Bovine Angiopoietin-2 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | ANGPT2 |
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| Gene Full Name | angiopoietin 2 |
| Alias Symbols | ANG-2, angiopoietin-2. |
| NCBI Gene Id | 282141 |
| Protein Name | Angiopoietin-2 |
| Description of Target | Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating angiogenic signals mediated by ANGPT1. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (By similarity). |
| Uniprot ID | O77802 |
| Protein Accession # | NP_001092325 |
| Nucleotide Accession # | NM_001098855 |
