Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 4 antibody [C2C3], C-term (GTX101669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(GTX101669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX101669 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using alpha Actinin 4(GTX101669) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the 2018 in Nat Commun. PMID: 30258067