Acetyl CoA is a critical molecule in metabolism. Acetyl CoA is an important molecule in the citric acid cycle to be oxidized for energy production. Also, Acetyl CoA is a key molecule in the biogenic synthesis of acetylcholine, melatonin, heme, sesquiterpenes, polyenes and long-chain fatty acids, precursors to cholesterol and other sterols, flavenoids and other polyketides. Acetyl CoA is also the source of the acetyl group used in histone acetylation.

 The Acetyl-CoA Fluorometric Assay Kit provides a convenient and highly sensitive way for the quantification of Acetyl CoA level in variety of biological samples. In the assay, free CoA is quenched and then Acetyl CoA is converted to CoA. The CoA is reacted to produce NADH which interacts with PicoProbe to produce fluorescence (Ex = 535/Em = 587 nm). The assay can quantify 10 to 1000 pmol of Acetyl CoA in a variety of samples with detection limit ~0.4 µM.