In vitro efficacity testing - Cell Activation
Assays to measure immune cell activation using multiple readouts, alone or in combination
Cell Activation
Immune cell activation is critical for an effective immune response to disease or infection. Aragen routinely performs assays to measure immune cell activation using multiple readouts, alone or in combination. Cells can be activated by general means (e.g. PHA, ConA) or methods activating specific cell subsets (e.g. CD3/CD28 or anti-IGM). Measurements of activation can utilize activation marker expression (e.g. CD69, CD25, CD71, CD154), protein phosphorylation, intracellular or multiplexed cytokine secretion, lymphoproliferation (e.g. BrdU incorporation, CFSE tracing, Ki-67 or PCNA expression) and others. These assays are performed in human whole blood, PBMCs or purified subsets and mouse splenocytes or whole blood. Whole blood provide a particularly useful format for pharmacodynamic assays.
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