Store at 28°C

Next Day 28°C

UltraStable™ HRP Conjugate Stabilizer, Mammalian (1X) preserves the activity of HRPprotein conjugates during storage, reconstitution, and immunoassay use. This HRP stabilizer can be used to store concentrated stock HRP conjugates, reconstitute lyophilized conjugates, and dilute HRP conjugated antibodies to their useful working titer in ELISA tests and immunologybased techniques. HRP Conjugate Stabilizer is especially suited for ELISAs using antiIgG HRP conjugates within traditional antigendown or antibody sandwich formats. Additional components may be added to HRP Conjugate Stabilizer, Mammalian, 1X to optimize it for particular ELISA format compositions or for use in other immunological techniques. Antimicrobial agents are included to retard bacterial growth

1. Protect the HRP conjugate stabilizer from light. This may be done by covering the container with aluminum foil.
2. Add the HRPconjugated protein at the target concentration to HRP Conjugate Stabilizer. For example, if diluting an HRPconjugated antibody 1:5000, add 10 μL conjugate stock to 49.99 mL HRP Conjugate Stabilizer.
3. Mix for 15 minutes.
4. Use the diluted HRP conjugate in the immunoassay.
5. Store remaining HRP conjugate solution at 28°C. Protect from direct exposure to light.