Tubulin β (4D9) monoclonal antibody-HRP

Product Name :
Tubulin β (4D9) monoclonal antibody-HRP Background :
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da._x000D_Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Tubulin β (4D9) mAb detects endogenous levels of β-Tubulin protein. Immunogen :
Synthetic peptide, corresponding to amino acids 402-455 of Human Tubulin β. Conjugate :
HRP Modification :
Unmodified

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  Western blot (WB) analysis of Tubulin β (4D9) monoclonal antibody-HRP at 1:10000 dilution Lane1:The Brain tissue lysate of Rat(10ug) Lane2:The Brain tissue lysate of Mouse(10ug) Lane3:PC12 whole cell lysate(10ug) Lane4:BV2 whole cell lysate(10ug) Lane5:A549 whole cell lysate(10ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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