Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP Method)
Referencia E-BC-K225-M-96T
embalaje : 96T
Marca : Elabscience
Contact local distributor :
Teléfono : +1 850 650 7790
Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP Method) (E-BC-K225-M)
For research use only.
| Detection Principle | Fe3+ -TPTZ can be reduced by antioxidants and produce blue Fe2+ -TPTZ under acid condition. The antioxidant capacity of sample can be calculated by detection the absorbance value at 593 nm. |
| Synonyms | T-AOC |
| Sample Type | Serum,Plasma,Saliva,Urine,Tissue,Cell,Cell culture supernatant |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (590-600 nm) |
| Research Area | Oxidative Stress , Plant Physiology Series |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.049 mmol/L |
| Detection Range | 0.049-2.5 mmol/L |
| Precision | inter-assay CV: 8.1% | intra-assay CV: 3.9% |
| Sample Volume | 5 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human saliva | 1 |
| Human urine | 1 |
| Cellular supernatant | 1 |
| HepG2 cells homogenization | 1 |
| 5% Mouse liver tissue homogenization | 1 |
| 10% Epipremnum aureum tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
No files available. Please enter the correct identification code in the search area to query the relevant documents.
| Title | Status | Valid until | Action | |
|---|---|---|---|---|
| {{v.文件名}} | {{v.status_str}} | {{v.timestamp_str}} | Download | / |
*Note：Please download the relevant files within the validity period. After expiration, file preview and download will not be available on the page.
Contact with Us
Houston, Texas, 77079, USA
