OCOA10408 | TMBIM4 Protein Lysate Quimigen Portugal

TMBIM4 Protein Lysate (OCOA10408)

Datasheets/Manuals	Printable datasheet for TMBIM4 Protein Lysate (OCOA10408)

Product Info
Predicted Species Reactivity	Homo sapiens
Product Format	Lyophilized
Host	HEK293T
Additional Information	Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::	Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and Storage	2°C to 8°C
Tag	C-MYC/DDK

Gene Symbol	TMBIM4
Gene Full Name	transmembrane BAX inhibitor motif containing 4
Alias Symbols	CGI-119, GAAP, Golgi anti-apoptotic protein, LFG4, protein lifeguard 4, Protein S1R, S1R, transmembrane BAX inhibitor motif-containing protein 4, Z-protein, ZPRO.
NCBI Gene Id	51643
Protein Name	Homo sapiens transmembrane BAX inhibitor motif containing 4 (TMBIM4), transcript variant 2, mRNA\|Protein lifeguard 4\|protein lifeguard 4 isoform b [Homo sapiens]
Description of Target	Anti-apoptotic protein which can inhibit apoptosis induced by intrinsic and extrinsic apoptotic stimuli. Can modulate both capacitative Ca2+ entry and inositol 1,4,5-trisphosphate (IP3)-mediated Ca2+ release.
Uniprot ID	Q9HC24
Protein Accession #	https://www.ncbi.nlm.nih.gov/protein/NP_057140
Nucleotide Accession #	https://www.ncbi.nlm.nih.gov/nuccore/NM_016056
Molecular Weight	28.85 kDa