SRSF1 ELISA Kit (Chicken)
Referencia OKEH06685
embalaje : 96Wells
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
SRSF1 ELISA Kit (Chicken) (OKEH06685)
| Datasheets/Manuals | Printable datasheet for SRSF1 ELISA Kit (Chicken) (OKEH06685) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Srsf1 ELISA Kit (Chicken) (OKEH06685) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Srsf1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Srsf1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Srsf1 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/ml | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
| Sensitivity | 0.097 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Chicken Serine/arginine-rich splicing factor 1 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | SRSF1 |
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| Gene Full Name | serine and arginine rich splicing factor 1 |
| Alias Symbols | serine/arginine-rich splicing factor 1, SFRS1, Splicing factor, arginine/serine-rich 1, splicing factor, arginine/serine-rich 1 (splicing factor 2, alternate splicing factor). |
| NCBI Gene Id | 772264 |
| Protein Name | Serine/arginine-rich splicing factor 1 |
| Description of Target | May play a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. |
| Uniprot ID | Q5ZML3 |
| Protein Accession # | NP_001107213 |
| Nucleotide Accession # | NM_001113741 |
