Western Blot
Western blot analysis of SARS-CoV-2 M polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 1: 293F cell lysate (negative control), Lane 2: 293F cell transfection pcDNA3.0-SARS-CoV-2 M-His. Western blot analysis of His-tag polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 3: 293F cell lysate, Lane 4: 293F cell transfection pcDNA3.0-SARS-CoV-2 M-His.
- Specifications
Product Description
Rabbit polyclonal antibody raised against partial synthetic peptide of SARS-CoV-2 M.
Immunogen
A synthetic peptide corresponding to amino acids 161-210 of SARS-CoV-2 M.
Host
Rabbit
Reactivity
SARS-CoV-2
Specificity
The antibody detects endogenous levels of SARS-CoV-2 M.
Form
Liquid
Purification
Affinity purification
Purity
> 95% by SDS-PAGE
Isotype
IgG
Recommend Usage
ELISA
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.02% sodium azide).
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot
Western blot analysis of SARS-CoV-2 M polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 1: 293F cell lysate (negative control), Lane 2: 293F cell transfection pcDNA3.0-SARS-CoV-2 M-His. Western blot analysis of His-tag polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 3: 293F cell lysate, Lane 4: 293F cell transfection pcDNA3.0-SARS-CoV-2 M-His. - Publication Reference
- Human iPSC-Derived Airway Models Enable Comparative Analysis of SARS-CoV-2 Infection in Healthy and COPD Bronchial Epithelium.
Lisa Morichon, Jitendriya Swain, Nathalie Gros, Amel Nasri, Florent Foisset, Gaetan Galisot, Victor Racine, Said Assou, Arnaud Bourdin, John De Vos, Delphine Muriaux.
iScience 2026 Jan; 29(3):114741.
Application：IF, Human, HY03, iCOPD2 cell.
- MERS-CoV and SARS-CoV-2 membrane proteins are modified with polylactosamine chains.
Dylan Juckel, Lowiese Desmarets, Adeline Danneels, Yves Rouillé, Jean Dubuisson, Sandrine Belouzard.
The Journal of General Virology 2023 Oct; 104(10).
Application：IF, WB, Human, Huh-7 cells.
- F-actin nanostructures rearrangements and regulation are essential for SARS-CoV-2 particle production in host pulmonary cells.
Jitendriya Swain, Peggy Merida, Karla Rubio, David Bracquemond, Aymeric Neyret, Israel Aguilar-Ordoñez, Stefan Günther, Guillermo Barreto, Delphine Muriaux.
iScience 2023 Jul; 26(8):107384.
Application：IF, Human, A549-hACE2 cells.
- Human iPSC-Derived Airway Models Enable Comparative Analysis of SARS-CoV-2 Infection in Healthy and COPD Bronchial Epithelium.