Recombinant Human IL-3
Referencia GMP-CF63-1mg
embalaje : 1mg
Marca : Novoprotein
Contact local distributor :
Teléfono : +1 850 650 7790
Description - Interleukin-3 (IL-3), also known as a multicolony-stimulating factor, is one of the important members of the interleukin family. It is mainly produced by activated T cells, macrophages, and bone marrow stromal stem cells. IL-3 plays an important role in hematopoietic regulation and immune regulation by binding to its receptor (IL-3R).
- IL-3 can not only induce the proliferation and maturation of pluripotent hematopoietic progenitor cells, bone marrow cells, eosinophils, basophilic cells, erythroid cells, and megakaryocytes, but also activate the cytotoxic, bactericidal, and anti-tumor activities of macrophages. Furthermore, IL-3 can also mobilize the response of cells (such as peripheral blood stem cells) to later activated cytokines such as G-CSF, thrombopoietin, and erythropoietin.
- Recombinant Human IL-3 is produced by E.coli expression system, and is produced with raw materials of pharmaceutical applicable level. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.
Usage In-vitro cultivation of various types of cells.
Quality Control | Item | Standard |
|---|---|
| Appearance | White porosity |
| Reconstitution | Totally dissolve in stated time |
| Visible particle | Conform to ChP |
| pH value | 6.9–7.9 |
| Purity | ≥ 95% |
| Bioactivity | ≥ 1.0×107 IU/mg |
| Endotoxin | < 10 EU/mg |
| Host protein | ≤ 0.005% |
| Exogenous DNA residue | ≤ 10 ng/mg |
| Mycoplasma test | Negative |
| Sterility test | Conform to ChP |
Storage Storage at -20±5℃, expiration date labeled on bottle.
Recommendations - （1）Make sure the product properly stored and none expired before using.
- （2）Please use water for injection for reconstitution, to a concentration > 100 μg/ml.
- （3）Sterile condition without pyrogen is required for reconstitution, re-aliquoting, dilution.
- （4）After reconstitution, aliquoted solution vials should be stored at -70°C and minimize freeze-thaw cycles preventing denaturation of protein.
Bioactivity-Cell Based Assay 
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.The specific activity of Recombinant Human IL-3 is ≥1.5 x 106 IU/mg, which is calibrated against the human IL-3 Standard (NIBSC code: 91/510).
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.