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> Rabbit Anti-NARS Polyclonal Antibody

Rabbit Anti-NARS Polyclonal Antibody

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Referencia HPA040017-25ul

embalaje : 25ul

Marca : Atlas Antibodies

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HPA040017
asparaginyl-tRNA synthetase

Anti-NARS Antibody

Polyclonal Antibody against HUMAN NARS

0.3 mg/ml
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
Anti-NARS Antibody
info@quimigen.pt

Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Product details

  • Target protein: asparaginyl-tRNA synthetase
  • Target gene: NARS
  • Verified species reactivity: Human, Mouse, Rat
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000024587 (91%)RAT - ENSRNOG00000017852 (88%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000024533
  • Concentration: 0.3 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:5000 - 1:10000
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC) 
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: asparaginyl-tRNA synthetase
  • Gene name: NARS
  • Alternative gene names: NARS1, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (1)

  • This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
    Human Protein Atlas