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Rabbit Anti-ETV3 Polyclonal Antibody

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Teléfono : +1 850 650 7790

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Teléfono : +1 850 650 7790

HPA004794

ets variant 3

Anti-ETV3 Antibody
- ChIP-Certified

Polyclonal Antibody against HUMAN ETV3

0.3 mg/ml

info@quimigen.pt

Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.

Target protein: ets variant 3
Target gene: ETV3
Verified species reactivity: Human
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000003382 (76%)RAT - ENSRNOG00000043095 (79%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Chromatin Immunoprecipitation (ChIP) recommended conditions
- Working concentration: 1-10µg per reaction
Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:50 - 1:200
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Chromatin Immunoprecipitation (ChIP)Validated for use in ChIP-Exo-Seq and other ChIP-based assays via chromatin immunoprecipitation followed by λ exonuclease digestion and next-generation DNA sequencing to precisely map protein-DNA interactions at single base pair resolution. *Please note not all antibody batches are validated for use in ChIP applications.
Immunofluorescence in Cell Lines (ICC-IF)
Immunohistochemistry (IHC)
Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Villar J, Cros A, De Juan A, Alaoui L, Bonte PE, Lau CM, Tiniakou I, Reizis B, Segura ENat Immunol , 2022 Dec 21; 24(1):84-95. Epub 2022 Dec 212022 Dec 21
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Human Protein Atlas