Reactivos e instrumentos para inmunología, biología celular y biología molecular.
 
> Rabbit Anti-ATP5B Polyclonal Antibody

Rabbit Anti-ATP5B Polyclonal Antibody

Imprimir Imprimir
Por favor conéctese

Referencia HPA001528-25ul

embalaje : 25ul

Marca : Atlas Antibodies

Contact local distributor :


Teléfono : +1 850 650 7790

HPA001528
ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide

Anti-ATP5B Antibody

Polyclonal Antibody against HUMAN ATP5B

0.1 mg/ml
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
Anti-ATP5B Antibody
info@quimigen.pt

Immunohistochemical staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody HPA001528 (A) shows similar protein distribution across tissues to independent antibody HPA001520 (B).

Product details

  • Target protein: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
  • Target gene: ATP5B
  • Verified species reactivity: Human, Mouse, Rat
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000025393 (99%)RAT - ENSRNOG00000002840 (100%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000011269
  • Concentration: 0.1 mg/ml

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunohistochemistry (IHC)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody   
  • Western Blot (WB)Genetic validation in WB by siRNA knockdown. Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
  • Gene name: ATP5B
  • Alternative gene names: ATPSB, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (6)

  • Lin HY, Liang CJ, Yang MY, Chen PL, Wang TM, Chen YH, Shih YH, Liu W, Chiu CC, Chiang CK, Lin CS, Lin HCApoptosis , 2024 Jan 28; 29(5-6):620-634. Epub 2024 Jan 282024 Jan 28
  • Nasca A, Mencacci NE, Invernizzi F, Zech M, Keller Sarmiento IJ, Legati A, Frascarelli C, Bustos BI, Romito LM, Krainc D, Winkelmann J, Carecchio M, Nardocci N, Zorzi G, Prokisch H, Lubbe SJ, Garavaglia B, Ghezzi DBrain , 2023 Mar 1; 146(7):2730-2738. Epub 2023 Mar 12023 Mar 1