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Porcine IL-8 ELISA Kit
Porcine IL-8 ELISA Kit
RayBio® Porcine (pig) IL-8 (CXCL8) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Porcine (pig) |
| Sensitivity | 10 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 10 pg/ml - 2000 pg/ml |
| Specificity | The sandwich ELISA antibody pair detects Porcine IL-8. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P26894 |
| Gene Id | 100620730|396880 |
| Gene Symbols | CXCL8, IL8 |
| Protein Name / Synonyms | Interleukin-8 (IL-8) (Alveolar macrophage chemotactic factor I) (AMCF-I) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Porcine IL-8 qIPCR ELISA Kit
As low as $639.00
RayPlex® Porcine IL-8 Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Porcine IL-8 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-8 (IL8 / CXCL8), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Effect of Pre-Exposure to Deoxynivalenol on the Response of Porcine Intestinal Epithelial Cells to F18 E. coli Infection
- Species:
- Porcine
- Sample Type:
- Cell Culture Supernatant (filtered cell culture media)
- Year:
- 2026
- Journal Name:
- Toxins
- PubMed ID:
- 41893564
Effects of dietary medium-chain fatty acid supplementation levels on growth performance, fecal score, energy, antioxidant, inflammatory status, and fecal metabolites of nursery pigs fed high-protein diets
- Species:
- Porcine
- Sample Type:
- Serum (nursery pigs, MCFA diets)
- Year:
- 2026
- Journal Name:
- Journal of Animal Science
- PubMed ID:
- 42528260
Highly pathogenic porcine reproductive and respiratory syndrome virus-induced inflammatory response in porcine pulmonary microvascular endothelial cells and effects of herbal ingredients on main inflammatory molecules
- Species:
- Pig
- Sample Type:
- porcine pulmonary microvascular endothelial cells (MVECs)
- Year:
- 2023
- Journal Name:
- International Immunopharmacology
Nucleocapsid protein residues 35, 36, and 113 are critical sites in up-regulating the Interleukin-8 production via C/EBPα pathway by highly pathogenic porcine reproductive and respiratory syndrome virus
- Species:
- Porcine
- Sample Type:
- porcine alveolar macrophages (PAMs)
- Year:
- 2023
- Journal Name:
- Microbial Pathogenesis
Synergistic Effect of Treatment with Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Lipopolysaccharide on the Inflammatory Response of Porcine Pulmonary Microvascular Endothelial Cells
- Species:
- Pig
- Sample Type:
- microvascular endothelial cells (MVECs)
- Year:
- 2023
- Journal Name:
- Viruses-basel
Concentrations of selected immunological parameters in the serum and processing fluid of suckling piglets and the serum and colostrum of their mothers
- Species:
- Porcine
- Sample Type:
- Other Body Fluid (processing fluid, colostrum); Serum
- Year:
- 2024
- Journal Name:
- BMC Veterinary Research
- PubMed ID:
- 38702674
Comparison of Host Cytokine Response in Piglets Infected With Toxigenic and Non-toxigenic Staphylococcus hyicus
- Species:
- Porcine
- Sample Type:
- Serum (S. hyicus-infected piglets)
- Year:
- 2021
- Journal Name:
- Frontiers in Veterinary Science
- PubMed ID:
- 33665221
Adenosine A 2A agonist improves lung function during ex vivo lung perfusion.
- Species:
- Pig
- Sample Type:
- Tissue Lysate (lung)
- Year:
- 2011
- Journal Name:
- The Annals of thoracic surgery
Gene expression profile suggests that pigs (Sus scrofa) are susceptible to Anaplasma phagocytophilum but control infection
- Species:
- Porcine
- Sample Type:
- Serum (Anaplasma pahgocytophilum infection model)
- Year:
- 2012
- Journal Name:
- Parasites and Vectors
- PubMed ID:
- 22935149
