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> Phosphatase, Alkaline, Liver, Bone, Kidney (Akp2, ALPI, ALPL) (FITC)

Phosphatase, Alkaline, Liver, Bone, Kidney (Akp2, ALPI, ALPL) (FITC)

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Referencia P4071-18-FITC-100ul

embalaje : 100ul

Marca : US Biological

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P4071-18-FITC Phosphatase, Alkaline, Liver, Bone, Kidney (Akp2, ALPI, ALPL) (FITC)

Clone Type
Monoclonal
Host
mouse
Source
human
Isotype
IgG1
Grade
Affinity Purified
Applications
FC IC IF IP
Crossreactivity
Fe Hu Mk Po Rb
Shipping Temp
Blue Ice
Storage Temp
-20°C

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This antibody can be used to monitor the expression of the human Liver/Bone/Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by Flow Cytometry.

Applications:
Suitable for use in Immunoprecipitation, Flow Cytometry, Immunocytochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Positive Control:
Undifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H414 (TRA-2-49/6E)|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein A affinity chromatography from ascites.|Immunogen: 2102Ep human Embryonal Carcinoma Cells. Epitope: Liver/Bone/Kidney Alkaline Phosphatase|Specificity: Recognizes human Liver/Bone/Kidney isozyme of Alkaline Phosphatase (AP). Does not crossreact with other isoforms of alkaline phosphatase. Species Crossreactivity: porcine, feline, tiger, rabbit, higher primates (chimpanzee, gorilla, orangutan, gibbon), and New World monkeys (owl monkey, squirrel monkey). No crossreactivity has been observed with bovine, canine, goat, guinea pig, hamster, rat, mouse, sheep and Old World monkeys (baboon, Rhesus monkeys, mangabey, mandrill, Celebese black ape, African green monkey).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
2102Ep human Embryonal Carcinoma Cells. Epitope: Liver/Bone/Kidney Alkaline Phosphatase
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography from ascites.
Specificity
Recognizes human Liver/Bone/Kidney isozyme of Alkaline Phosphatase (AP). Does not crossreact with other isoforms of alkaline phosphatase. Species Crossreactivity: porcine, feline, tiger, rabbit, higher primates (chimpanzee, gorilla, orangutan, gibbon), and New World monkeys (owl monkey, squirrel monkey). No crossreactivity has been observed with bovine, canine, goat, guinea pig, hamster, rat, mouse, sheep and Old World monkeys (baboon, Rhesus monkeys, mangabey, mandrill, Celebese black ape, African green monkey).
References
1. Andrews, P.W., et al., Hybridoma 3: 33-39 (1984). 2. Swallow, D.M., et al., Ann. Hum. Genet. 50: 229-235 (1986). 3. Draper, J.S., et al., J. Anat. 200: 249-258 (2002). 4. Henderson, J.K., et al., Stem Cells 20: 329-337 (2002). 5. Andrews P.W., et al Int. J.Cancer. 66(6): 806-816 (1996).