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- Product Name
- PE Anti-Mouse IL-10 Antibody(JES5-16E3)
- Catalogue No.
- PE-30146
- Form
- liquid
- Conjugation
- PE
- Conjugation Information
- PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
- Clonality
- monoclonal
- Isotype
- IgG2b, kappa
- Clone ID
- JES5-16E3
- Storage
- PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- interleukin 10
- Alternative Names
- Interleukin-10 (IL-10)|Cytokine synthesis inhibitory factor (CSIF)|IL10 antibody
- UniProt ID
- P18893
Application
- Tested Applications
- FC
- Recommended dilution
- Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Validated Images
HEK293T cells transiently transfected with pcDNA3.1 plasmid encoding Mouse IL-10 gene are stained with PE Anti-Mouse IL-10 Antibody (filled red histogram) or PE Rat IgG2b, κ lsotype Control (empty black histogram). - Background
- The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.