NGF ELISA Kit (Rat)
Referencia OKEH00187
embalaje : 96Wells
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
NGF ELISA Kit (Rat) : 96 Wells (OKEH00187)
| Datasheets/Manuals | Printable datasheet for NGF ELISA Kit (Rat) : 96 Wells (OKEH00187) |
|---|---|
| COA Datasheet | Printable COA datasheet for OKEH00187 |
| Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology NGF ELISA Kit (Rat) (OKEH00187) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NGF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NGF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NGF captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 15.6-1000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 5.3% (n = 20) Mean Inter-assay CV%: < 8.2% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 7.8 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Rat Beta-nerve growth factor | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Ngf |
|---|---|
| Gene Full Name | nerve growth factor |
| Alias Symbols | beta-nerve growth factor, beta-NGF, nerve growth factor (beta polypeptide), Ngfb, pro-nerve growth factor long. |
| NCBI Gene Id | 310738 |
| Protein Name | Beta-nerve growth factor |
| Description of Target | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI. |
| Uniprot ID | P25427 |
| Protein Accession # | XP_006233115 |
| Nucleotide Accession # | XM_006233053 |
| Protein Size (# AA) | 241 |
| Molecular Weight | 27 kDa |
