NALM-6 cells
Referencia C0003030
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Disease:
Acute lymphoblastic leukemia (ALL)
Origin:
The line was established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukemia (ALL) in relapse in 1976.
Species:
Homo sapiens, human Peripheral blood Lymphocyte-like Single, small, round cells in suspension human near diploid karyotype - 46(43-47)<2n>XY, t(5;12)(q33.2;p13.2) Male, 19 years of age. AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS Cultures can be maintained by addition or replacement of fresh medium. Start cultures between 2 X 10^5 cells/mL and 4 X 10^5cells/mL maintain between 3 X 10^5 and 3 X 10^6 cells/mL. Culture at 5% CO2, 37°C. Complete growth culture medium supplemented with 7.5% (v/v) DMSO I Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative Amelogenin: X,Y CSF1PO: 12 D13S317: 9,11 D16S539: 11 D5S818:11 D7S820: 8,12 THO1:8,9 TPOX: 8,10 vWA: 15,18 If use of this culture results in a scientific publication, it should be cited in the publication as: NALM-6 (AddexBio C0003030) Tissue:
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Citation Format:
References for NALM-6 cells:
1. Hurwitz R, Hozier J, LeBien T, Minowada J, Gajl-Peczalska K, Kubonishi I, Kersey J. Characterization of a leukemic cell line of the pre-B phenotype. Int J Cancer. 1979 Feb;23(2):174-180. PMID: 83966