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> N-Acyl Mannosamine-1-Dehydrogenase (Biotin)

N-Acyl Mannosamine-1-Dehydrogenase (Biotin)

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Referencia N0008-02-100ug

embalaje : 100ug

Marca : US Biological

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N0008-02 N-Acyl Mannosamine-1-Dehydrogenase (Biotin)

Clone Type
Polyclonal
Host
goat
Isotype
IgG
Grade
Highly Purified
Applications
DB E IHC WB
Crossreactivity
Gt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for Immunoblot (Western or Dot Blot), ELISA, Immunomicroscopy and Immunohistochemistry as well as other antibody based assays using streptavidin or avidin conjugates. Other applications not tested.

Recommended Dilution:
ELISA: 1:7000-1:35,000; 1ug of N-Acylmannosamine-1-Dehydrogenase was used in a standard capture ELISA with Streptavidin (HRP) S7973-97A and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]), A0030, as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.

Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)

Biotin/Protein Ratio:
10-20 BAC molecules per Goat IgG molecule

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Concentration: ~10mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol. Labeled with Biotin.|Purity: Purified by delipidation, salt fractionation and ion exchange chromatography.|Immunogen: N-Acylmannosamine-1-Dehydrogenase (Pseudomonas).|Specificity: Assay by IEP resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified N-Acylmannosamine-1-Dehydrogenase (Pseudomonas).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
N-Acylmannosamine-1-Dehydrogenase (Pseudomonas).
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol. Labeled with Biotin.
Purity
Purified by delipidation, salt fractionation and ion exchange chromatography.
Specificity
Assay by IEP resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified N-Acylmannosamine-1-Dehydrogenase (Pseudomonas).
References
Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.