Multiplex ELISA Kit For Porcine Cytokine Array (4-plex)
Referencia MEK1018
embalaje : 1kit
Marca : Boster Bio
Contact local distributor :
Teléfono : +1 850 650 7790
Product Info Summary
| Catalog: | MEK1018 |
|---|---|
| Species: | Porcine |
| Sample Type: | Serum, Plasma |
| Volume Required per Well: | Min 25 µl |
| Assay Length: | 4.83 hours (290 minutes) |
| Specificity: | All analytes < 1% intra-panel cross-reactivity |
| Assay Type: | Planar Based Multiplexed ELISA |
| Detection Method: | Chemiluminescent |
| Multiplex Format: | 96-well solid plate |
| Manufacturer Reference Number: | 119149PC |
| Analytes: | IL-1β, IL-6, IL-8, TNFα |
Product Overview
The Multiplex Porcine Cytokine – High Sensitivity (4-plex) is a fully quantitative, ELISA-based chemiluminescent assay allowing concurrent measurement of cytokines in serum or plasma samples.
A kit contains a 96-well plate, with each well featuring the 4-plex along with a positive control with all the other reagents necessary to perform the test. Our high-quality reagents help ensure the accuracy of your results.
Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 4.83 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
Assay Ranges
| Analytes | Calibrator Range | Units | LLD |
|---|---|---|---|
| IL-1β | 1550 - 2.13 | pg/mL | 1.06 |
| IL-6 | 1800 - 2.47 | pg/mL | 1.23 |
| IL-8 | 2100 - 2.88 | pg/mL | 1.44 |
| TNFα | 1600 - 2.19 | pg/mL | 1.10 |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with blockers and preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips