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Mouse VEGF ELISA Kit
Mouse VEGF ELISA Kit
RayBio® Mouse VEGF-A ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Mouse |
| Sensitivity | 2 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 pg/ml - 1000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 3 fold |
| Accession Number | Q00731 |
| Gene Id | 22339 |
| Gene Symbols | VEGFA, VEGF |
| Protein Name / Synonyms | Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cardiovascular Disease, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
Related Products
NexaTag® Mouse VEGF qIPCR ELISA Kit
As low as $639.00
RayPlex® Mouse VEGF-A Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Mouse VEGF ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified VEGFA, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Trem2 acts as a non-classical receptor of interleukin-4 to promote diabetic wound healing
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (supernatants from anti-inflammatory macrophage cultures)
- Year:
- 2024
- Journal Name:
- Clinical and Translational Medicine
- PubMed ID:
- 39350473
Enterococcus faecalis promotes the progression of colorectal cancer via its metabolite: biliverdin
- Species:
- Mouse
- Sample Type:
- HCT116 cell-xenograft, SW480 cell-xenograft
- Year:
- 2023
- Journal Name:
- Journal of Translational Medicine
Enhancing alginate dialdehyde-gelatin (ADA-GEL) based hydrogels for biofabrication by addition of phytotherapeutics and mesoporous bioactive glass nanoparticles (MBGNs)
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (cell culture medium of MC3T3-E1 cells)
- Year:
- 2025
- Journal Name:
- Journal of biomaterials applications
- PubMed ID:
- 39305217
Murine Cell Line Models for Vascular Mimicry: The Role of YAP/TAZ Signaling
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (normoxic or hypoxic culture)
- Year:
- 2025
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 41009691
A 3D-Printed Wound-Healing Material Composed of Alginate Dialdehyde–Gelatin Incorporating Astaxanthin and Borate Bioactive Glass Microparticles
- Species:
- Mouse
- Sample Type:
- fibroblast
- Year:
- 2023
- Journal Name:
- Acs Applied Materials & Interfaces
Novel lipid mediator 7S,14R-docosahexaenoic acid: biogenesis and harnessing mesenchymal stem cells to ameliorate diabetic mellitus and retinal pericyte loss
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (MSC culture supernatant)
- Year:
- 2024
- Journal Name:
- Frontiers in Cell and Developmental Biology
- PubMed ID:
- 38533089
Microglia-Derived Olfactomedin-like 3 Is a Potent Angiogenic Factor in Primary Mouse Brain Endothelial Cells: A Novel Target for Glioblastoma
- Species:
- Mouse
- Sample Type:
- endothelial cells (ECs)
- Year:
- 2022
- Journal Name:
- International Journal of Molecular Sciences
Activated androgen receptor accelerates angiogenesis in cutaneous neurofibroma by regulating VEGFA transcription
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (conditioned medium CM from shNf1-SW10 cells, shNf1 fibroblasts)
- Year:
- 2019
- Journal Name:
- International Journal of Oncology
- PubMed ID:
- 31059067
A self-designed CpG ODN enhanced the anti-melanoma effect of pimozide
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Peripheral blood)
- Year:
- 2020
- Journal Name:
- International Immunopharmacology
The Effects of Simulated Microgravity on Macrophage Phenotype
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (culture media from RAW 264.7 macrophages)
- Year:
- 2021
- Journal Name:
- Biomedicines
- PubMed ID:
- 34572391
