Mouse Phosphorylated Smad 2 (pSmad3) ELISA Kit
Referencia MBS269937-96
embalaje : 96-Strip-Wells
Marca : MyBiosource
Contact local distributor :
Teléfono : +1 850 650 7790
Phosphorylated Smad 2 (pSmad3) ELISA Kit for Mouse
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS269937Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse pSmad3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and… More details
Product Overview
Online Datasheet in PDFProduct Name
Phosphorylated Smad 2 (pSmad3), ELISA Kit
Full Product Name
Mouse Phosphorylated Smad 2 (pSmad3) ELISA Kit
Product Gene Name
pSmad3 elisa kit
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Samples
Mouse serum, plasma or cell culture supernatant
Detection Range
20 ng/ml-0.312 ng/ml
Sensitivity
Up to 0.06 ng/ml
Specificity
No cross-reaction with other factors.
Preparation and Storage
Store all reagents at 2-8 degree C.
Inter-assay Precision
<= 12%
Intra-assay Precision
<= 8%
Molecular Weight
42,417 Da
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of pSmad3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for pSmad3 purchase
MBS269937 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphorylated Smad 2 (pSmad3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing pSmad3. The ELISA analytical biochemical technique of the MBS269937 kit is based on pSmad3 antibody-pSmad3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect pSmad3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, pSmad3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for pSmad3. It may not necessarily be applicable to this product.
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein phosphatase 1A
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent, 1A
NCBI Official Symbol
Ppm1a
NCBI Official Synonym Symbols
Pp2c1
UniProt Protein Name
Protein phosphatase 1A
UniProt Synonym Protein Names
Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA
UniProt Gene Name
Ppm1a
UniProt Synonym Gene Names
Pp2c1; Pppm1a; PP2C-alpha
UniProt Entry Name
PPM1A_RAT
NCBI Protein Information
protein phosphatase 1A; IA; PP2C-alpha; protein phosphatase IA; protein phosphatase 2C isoform alpha; protein phosphatase 1A, magnesium dependent, alpha isoform; Protein phosphatase type 1A (formely 2C) Mg-dependent alpha isoform; Protein phosphatase type 1A (formely 2C), Mg-dependent, alpha isoform
UniProt Comments for pSmad3
PPPM1A: a Ser/Thr protein phosphatase of the PP2C family. Inhibits the activation of p38 and JNK kinase cascades induced by environmental stresses. Can also dephosphorylate cyclin-dependent kinases and may play a role in cell cycle control. Overexpression of this phosphatase activates the expression of p53, leading to G2/M cell cycle arrest and apoptosis.
Protein type: Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.16
Cellular Component: neuron projection; membrane; voltage-gated calcium channel complex; nucleus; cytosol
Molecular Function: protein C-terminus binding; protein binding; signal transducer activity; calmodulin-dependent protein phosphatase activity; manganese ion binding; magnesium ion binding; protein serine/threonine phosphatase activity; phosphoprotein phosphatase activity
Biological Process: positive regulation of protein export from nucleus; N-terminal protein myristoylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; Wnt receptor signaling pathway; dephosphorylation; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transforming growth factor beta receptor signaling pathway; protein amino acid dephosphorylation; positive regulation of Wnt receptor signaling pathway; negative regulation of BMP signaling pathway
Show more Protein type: Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.16
Cellular Component: neuron projection; membrane; voltage-gated calcium channel complex; nucleus; cytosol
Molecular Function: protein C-terminus binding; protein binding; signal transducer activity; calmodulin-dependent protein phosphatase activity; manganese ion binding; magnesium ion binding; protein serine/threonine phosphatase activity; phosphoprotein phosphatase activity
Biological Process: positive regulation of protein export from nucleus; N-terminal protein myristoylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; Wnt receptor signaling pathway; dephosphorylation; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transforming growth factor beta receptor signaling pathway; protein amino acid dephosphorylation; positive regulation of Wnt receptor signaling pathway; negative regulation of BMP signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with pSmad3 elisa kit
| Reference Product | PubMed Publications |
|---|---|
| Smad2 elisa kit | >13 publications with pSmad3 and Smad2 |
| Smad3 elisa kit | >10 publications with pSmad3 and Smad3 |
| Smad4 elisa kit | >1 publications with pSmad3 and Smad4 |
Pathways associated with pSmad3 elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Disease Pathway antibodies | Disease Pathway Diagram |
| Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway antibodies | Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway Diagram |
| Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway antibodies | Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway Diagram |
| Gene Expression Pathway antibodies | Gene Expression Pathway Diagram |
| Generic Transcription Pathway antibodies | Generic Transcription Pathway Diagram |
| IGF1R Signaling Cascade Pathway antibodies | IGF1R Signaling Cascade Pathway Diagram |
| IRS-mediated Signalling Pathway antibodies | IRS-mediated Signalling Pathway Diagram |
| IRS-related Events Pathway antibodies | IRS-related Events Pathway Diagram |
| IRS-related Events Triggered By IGF1R Pathway antibodies | IRS-related Events Triggered By IGF1R Pathway Diagram |
| Insulin Receptor Signalling Cascade Pathway antibodies | Insulin Receptor Signalling Cascade Pathway Diagram |
Diseases associated with pSmad3 elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Necrosis Antibodies | >5 publications with pSmad3 and Necrosis |
| Fibrosis Antibodies | >4 publications with pSmad3 and Fibrosis |
| Disease Models, Animal Antibodies | >2 publications with pSmad3 and Disease Models, Animal |
| Kidney Diseases Antibodies | >2 publications with pSmad3 and Kidney Diseases |
| Adenocarcinoma Antibodies | >1 publications with pSmad3 and Adenocarcinoma |
| Depressive Disorder Antibodies | >1 publications with pSmad3 and Depressive Disorder |
| Neoplasm Metastasis Antibodies | >1 publications with pSmad3 and Neoplasm Metastasis |
| Carcinoma, Hepatocellular Antibodies | >1 publications with pSmad3 and Carcinoma, Hepatocellular |
| Liver Neoplasms Antibodies | >1 publications with pSmad3 and Liver Neoplasms |
| Inflammation Antibodies | >1 publications with pSmad3 and Inflammation |
Organs/Tissues associated with pSmad3 elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Embryonic Tissue Antibodies | >4 publications with pSmad3 and Embryonic Tissue |
| Liver Antibodies | >2 publications with pSmad3 and Liver |
| Eye Antibodies | >2 publications with pSmad3 and Eye |
| Kidney Antibodies | >2 publications with pSmad3 and Kidney |
| Connective Tissue Antibodies | >2 publications with pSmad3 and Connective Tissue |
| Placenta Antibodies | >2 publications with pSmad3 and Placenta |
| Brain Antibodies | >2 publications with pSmad3 and Brain |
| Ovary Antibodies | >1 publications with pSmad3 and Ovary |
Usted podría estar interesado también en los siguientes productos:
Referencia
Descripción
Cond.
Precio Sin IVA
