Mouse IgM Antibody : HRP
Referencia OASB00176
embalaje : 1.0mL
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
Mouse IgM Antibody : HRP (OASB00176)
| Datasheets/Manuals | Printable datasheet for Mouse IgM Antibody : HRP (OASB00176) |
|---|
| Tested Species Reactivity | Mouse |
|---|---|
| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | SB73a |
| Isotype | IgG1 |
| Host | Rat |
| Conjugation | HRP |
| Application | IHC-P, WB |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse IgM hybridoma |
| Concentration | Lot specific |
| Specificity | IgM |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of astandard reference reagent using immunofluorescence staining and analysis by flow cytometry and/or enzymelinked immunosorbent assay (ELISA). |
| Dilution | Flow Cytometry: ELISA: Purified antibody Purified antibody Alkaline phosphatase conjugate Horseradish peroxidase conjugate <=1 ug/106 cells 1ug/mL 1:1,000-1:2,000 1:2,000-1:4,000 |
| Application Info | Identification and enumeration of IgM+ cells by flow cytometry, Identification and enumeration of IgM+ cells by immunofluorescence microscopy, Second step reagent for mouse IgM monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA) |
| Other Applications Data | Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C. |
