Use code ELISA25-262 for 25% off all ELISAs through November.
25% off all ELISA kits
Mouse G-CSF ELISA Kit
Mouse G-CSF ELISA Kit
RayBio® Mouse GCSF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Mouse |
| Sensitivity | 0.5 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.5 pg/ml - 150 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 20 - 100 fold |
| Accession Number | P09920 |
| Gene Id | 12985 |
| Gene Symbols | CSF3, CSFG |
| Protein Name / Synonyms | Granulocyte colony-stimulating factor (G-CSF) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Mouse G-CSF qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Mouse G-CSF ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified GCSF (CSF3), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Cholesterol efflux pathways hinder KRAS-driven lung tumor progenitor cell expansion
- Species:
- Mouse
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Cell Stem Cell
Intranasal Bifidobacterium longum protects against viral-induced lung inflammation and injury in a murine model of lethal influenza infection
- Species:
- Mouse
- Sample Type:
- Serum, BAL Fluid (cytokines)
- Year:
- 2020
- Journal Name:
- EBioMedicine
Maladaptive innate immune training of myelopoiesis links inflammatory comorbidities
- Species:
- Mouse
- Year:
- 2022
Cebpd Is Essential for Gamma-Tocotrienol Mediated Protection against Radiation-Induced Hematopoietic and Intestinal Injury
- Species:
- Mouse
- Sample Type:
- Plasma
- Year:
- 2018
- Journal Name:
- Antioxidants
Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (conditioned media from PDAC cell lines)
- Year:
- 2020
- Journal Name:
- Cell Reports
- PubMed ID:
- 31940491
Intravesical treatment of chemotherapeutic agents sensitizes bacillus Calmette‑Guerin by the modulation of the tumor immune environment
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2019
- Journal Name:
- Oncology Reports
- PubMed ID:
- 30628699
Myelopoiesis is regulated by osteocytes through Gs-dependent signaling
- Species:
- Mouse
- Sample Type:
- Conditioned Media (osteocyte-enriched bone explants); Serum (osteocyte-enriched bone explants)
- Year:
- 2013
- Journal Name:
- Blood
- PubMed ID:
- 23160461
Macrophage β2-Integrins Regulate IL-22 by ILC3s and Protect from Lethal Citrobacter rodentium-Induced Colitis
- Species:
- Mouse
- Sample Type:
- Serum (C. rodentium-infected CD18-/- mice)
- Year:
- 2019
- Journal Name:
- Cell Reports
- PubMed ID:
- 30726742
G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Mouse 4T1 cells in breast cancer model)
- Year:
- 2016
- Journal Name:
- Oncoimmunology
Succinate ester derivative of -tocopherol enhances the protective effects against 60Co -ray-induced hematopoietic injury through granulocyte colony-stimulating factor induction in mice
- Species:
- Mouse
- Sample Type:
- Serum (Tocopherol treatment to protect against irradiation (Co60))
- Year:
- 2017
- Journal Name:
- Scientific reports
