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> Mouse Anti-PDIA3 Monoclonal Antibody [Clone:CL2444]

Mouse Anti-PDIA3 Monoclonal Antibody [Clone:CL2444]

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Referencia AMAb90988

embalaje : 100ul

Marca : Atlas Antibodies

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Teléfono : +1 850 650 7790

AMAb90988
protein disulfide isomerase family A, member 3

Anti-PDIA3 Antibody

Monoclonal Antibody against HUMAN PDIA3

1.0 mg/ml
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
Anti-PDIA3 Antibody
info@quimigen.pt

Immunohistochemistry analysis in human thyroid gland and skeletal muscle tissues using AMAb90988 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.

Product details

  • Target protein: protein disulfide isomerase family A, member 3
  • Target gene: PDIA3
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000027248 (91%)RAT - ENSRNOG00000015018 (91%)
  • Clonality: Monoclonal
  • Clone ID: CL2444
  • Isotype: IgG1
  • Host: Mouse
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Protein A purified
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: MAB-03986
  • Concentration: 1.0 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 2-10 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:20000 - 1:50000
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 1 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  
  • Western Blot (WB)Genetic validation in WB by siRNA knockdown. Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: protein disulfide isomerase family A, member 3
  • Gene name: PDIA3
  • Alternative gene names: ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (12)

  • Ayupov T, Moreno-Juan V, Curtoni S, Fratzl A, Sharma U, Posada-Céspedes S, Ratiu R, Morikawa R, Meyer AG, Pezzoli M, Bantug G, Chevalier M, Hou Y, Nadeau SA, Herrero-Navarro Á, Ayinampudi V, Kastanaki E, Whitehead N, Siwicki RA, Ribeiro MM, Han JH, Bucci A, Hess C, Picelli S, Renner M, Müller DJ, Cowan CS, Hansen S, Roska BNature , 2026 May; 653(8113):221-231. Epub 2026 Apr 152026 May
  • Palma M, Chaufan M, Breuer CB, Müller S, Sabatier M, Fraser CS, Szylo KJ, Yavari M, Carmona A, Kaur M, Melo LM, Cansiz F, Monge-Lorenzo J, Flores M, Mishima E, Nakamura T, Proneth B, Labrado M, Liang Y, Cayting N, Zheng L, Cañeque T, Colombeau L, Wahida A, Friedmann Angeli JP, Tasdogan A, Hui S, Rodriguez R, Conrad M, Reticker-Flynn NE, Ubellacker JMNature , 2025 Nov 5; 649(8096):477-486. Epub 2025 Nov 52025 Nov 5

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