CD5, a 55kD cell surface antigen, otherwise known as the interleukin-2 (IL-2) receptor alpha chain. In rabbits, CD25 is expressed on activated T lymphocytes.

Mouse anti Rabbit CD25 antibody, is reported to inhibit the binding of IL-2 to its receptor (Kotani et al. 1993).

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
PAI myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.