Monoclonal Mouse anti-Rat PCNA Antibody (clone SPM350, Azide-free,BSA-free, IHC, IF, WB)
Referencia LS-C392374-100
embalaje : 100ug
Marca : LSBio
Contact local distributor :
Teléfono : +1 850 650 7790
Overview
Antibody:
PCNA Mouse anti-Rat Monoclonal (Azide-free, BSA-free) (SPM350) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Rat, Human, Monkey, Mouse, Pig, Chicken, Zebrafish, Drosophila, Yeast
Format:
Unconjugated, Azide-free, BSA-free
Unmodified
Specifications
Description
PCNA antibody LS-C392374 is an unconjugated mouse monoclonal antibody to PCNA from rat. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
Target
Rat PCNA
Synonyms
PCNA | Cyclin
Host
Mouse
Reactivity
Rat, Human, Monkey, Mouse, Pig, Chicken, Zebrafish, Drosophila, Yeast (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
SPM350
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free. Also available Unmodified.
Immunogen
Rat PCNA protein.
Specificity
Rat PCNA
Applications
- IHC
- IHC - Paraffin (0.25 - 0.5 µg/ml)
- Immunofluorescence (0.5 - 1 µg/ml)
- Western blot (0.5 - 1 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. The optimal dilution of the anti-PCNA antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Presentation
1X PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee About PCNA
P12004 NM_002592 NP_002583.1
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
tech@quimigen.pt