Methylene Blue trihydrate [7220-79-3]
Referencia NB-64-00142-10g
embalaje : 10g
Marca : Neo Biotech
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Teléfono : +1 850 650 7790
Methylene Blue trihydrate (Synonyms: Methylthionine chloride, Methylene Blue, Basic blue 9)
Catalog No. T0006 Copy Product Info
Purity: 99.89%
Methylene Blue trihydrate (Basic blue 9) is a compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene Blue trihydrate is used as a bacteriologic stain and as an indicator. It inhibits soluble guanylyl cyclase and has been used to treat cyanide poisoning and to lower levels of methemoglobin. It also inhibits tau filament formation (IC50: 1.9μM).
Methylene Blue trihydrate
Copy Product InfoSynonyms Methylthionine chloride, Methylene Blue, Basic blue 9
Methylene Blue trihydrate (Basic blue 9) is a compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene Blue trihydrate is used as a bacteriologic stain and as an indicator. It inhibits soluble guanylyl cyclase and has been used to treat cyanide poisoning and to lower levels of methemoglobin. It also inhibits tau filament formation (IC50: 1.9μM).
Cas No. 7220-79-3
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Purity:99.89%
Color:Green to Black
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Product Introduction
Methylene Blue trihydrate AI Summary
Methylene Blue trihydrate has a high solubility of 53.4 μM in a 100 mM phosphate buffer at pH 7.0, suggesting it is readily dissolved and available for biological activities in aqueous environments. The compound displays a spectrum of bioactivities, including cytotoxic effects on various cell lines, affecting cell viability and ATP levels. It inhibits Setaria cervi glutathione reductase with a 24% inhibition rate at concentrations between 2 to 200 μM. Additionally, it demonstrates inhibitory effects on pathways and enzymes related to DNA, hormone receptors, genomic imprints, and metabolic activity. Methylene Blue trihydrate exhibits significant antiviral activity against SARS-CoV-2, achieving a 99.16% inhibition rate at 10 μM concentration after 48 hours. It has a selectivity index of 4.0 for SARS-CoV-2 induced cytotoxicity in Caco-2 cells with an IC50 value of 2030.0 nM and a CC50 value of 7250.0 nM, suggesting a favorable safety profile. It inhibits native rhodanese and Escherichia coli GroEL with IC50 values of 6100.0 nM and 3300.0 nM, respectively, and inhibits ATPase activity of E. coli GroEL with an IC50 >250000.0 nM. Moreover, it shows 67% and 54% inhibition against the refolding activity of E. coli GroEL for rhodanese and pig heart malate dehydrogenase (MDH), respectively. Despite its broad antiviral and enzyme inhibitory activities, Methylene Blue trihydrate demonstrates inconsistent antiviral effects against SARS-CoV-2 3CL-Pro protease, with an inhibition percentage of -9.023% at a 20 μM concentration, indicating variable efficacy. However, it still showed some inhibition of SARS-CoV-2 induced cytotoxicity in VERO-6 cells..
Note: Summary generated by AI. Data source: ChEMBL 
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Methylene Blue trihydrate (Basic blue 9) is a compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene Blue trihydrate is used as a bacteriologic stain and as an indicator. It inhibits soluble guanylyl cyclase and has been used to treat cyanide poisoning and to lower levels of methemoglobin. It also inhibits tau filament formation (IC50: 1.9μM). |
| Targets&IC50 | Tau filament:1.9μM |
| Cell Research | Instructions 1. Cell and tissue staining 1. Dissolve methylene blue in an appropriate solvent (such as water or buffer) to prepare a dye solution of a certain concentration. 2. Soak the cells or tissue sections in the dye solution, usually the staining time is several minutes. 3. After cleaning, observe the cell structure or distribution of target molecules under a microscope. 2. As MAO-A inhibitor and antidepressant and anti-anxiety drugs Methods: Through animal models or cell culture systems, the regulatory effect on neurotransmitters was studied. III. Research on neurodegenerative diseases Methods: Through animal models and cell models, its neuroprotective effects were studied. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Synonyms | Methylthionine chloride, Methylene Blue, Basic blue 9 |
| Molecular Weight | 373.9 |
| Formula | C16H24ClN3O3S |
| Cas No. | 7220-79-3 |
| Smiles | O.O.O.[Cl-].CN(C)c1ccc2nc3ccc(cc3[s+]c2c1)N(C)C |
| Relative Density. | 0.98 g/cm3 at 25℃ |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||
| Solubility Information | DMSO: 3.75 mg/mL (10.03 mM), Sonication is recommended.  | ||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||